FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345091342
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG1558350.018.216371
GGGACCT1679950.018.179281
GAAATGA1042350.018.127751
CTGAAAC1290500.018.1063731
GAATGAA1053950.017.9575061
GTATGAA999450.017.560878
TACCTTA1689750.017.4954341
AAATGAG810450.017.4827752
TATGAAA503750.017.4303689
TGCGGGT1682200.017.3769341
TGAGCCG1788900.017.3452761
ATGAAAC1168100.017.34409710
GTAGCAA1290200.017.3384578
GGGAATG868650.017.2898521
CAATGAA707000.017.2428428
CGCGGAT1125650.017.2182259
AGAGCGA1023600.017.2152181
GTGGAAA804950.017.212228
AGCTGAA922700.017.1986878
AGACTAG1040200.017.1961278
Produced by FastQC (version 0.11.5)