FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345091342
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5737580.166262647064614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2365950.045.466459
AAGCAGT2996000.044.76451
GGTATCA2591400.040.9163138
TGGTATC2674550.038.4693537
GTGGTAT2741100.037.5321436
AGCAGTG3775000.035.6807442
CAGTGGT3590200.029.9894334
AGTGGTA3604150.029.632985
ATCAACG2808700.028.57274210-11
CAACGCA3278550.026.99860612-13
GCAGTGG5009800.025.5787243
ACGCAGA3374150.025.07422814-15
TCAACGC3013200.024.86090912-13
AGAGTAC2563200.023.45983318-19
AACGCAG3592100.022.17140214-15
CGCAGAG3912900.019.53416416-17
GCAGAGT3256050.019.32030916-17
CAGAGTA2980900.018.92243218-19
TATCAAC3227750.018.2394110-11
GTACTTT2449100.017.54142622-23
Produced by FastQC (version 0.11.5)