FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362741776
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1087100.018.208381
CAATGGA1674700.018.06468
CATCGAA1494450.017.9333441
TGCCAAA1266100.017.9312631
CTCGAAA748050.017.737141
ACTGATG1230750.017.5002231
GACTAAC847500.017.3998741
CTGATAG1091150.017.3375021
GGAAACA717950.017.22091110
GATGAAA680900.017.1750951
AGGGAAA721100.017.1448928
TGACTAG1050150.017.125671
TCTGGAA896150.017.0287911
TACACGA829950.017.0225331
AAACCTG1270150.017.0020661
GGGAAAC727650.016.9838459
GGGAATG879200.016.9220541
ACGAGGA795950.016.8774551
GGCTCGA900400.016.825621
TGACGGC1133700.016.798371
Produced by FastQC (version 0.11.5)