FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362741776
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1054250.034.377939
ATCAACG958700.026.7114310-11
CAACGCA1099600.024.9157512-13
GGTATCA1449800.024.8796928
TCAACGC1098350.022.4019812-13
TGGTATC1630500.021.5078627
AAGCAGT2076950.020.8915271
GTGGTAT1757050.020.1116836
AACGCAG1386250.018.40667714-15
ACGCAGA1511900.017.35529514-15
CTACACG270450.016.4332641
AGCAGTG2719750.016.2441392
AGAGTAC1266800.014.95425418-19
ACGACGC294950.014.8355815
TATCAAC1425100.013.75531710-11
CAGTGGT2952400.012.7260864
CGCAGAG1945400.012.461452516-17
TACACGA361500.012.164282
AGTGGTA3520700.011.1870855
GCAGTGG4221700.010.5297333
Produced by FastQC (version 0.11.5)