FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350348654
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC4614450.13171022486645545No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT3641940.10395187646418075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGGG1698300.017.7651910
AGAAATG886250.017.63845310
CGGACTG1372600.017.602921
GCCTCTA3589700.017.5881331
AGGGTGA2774200.017.5715771
AACTGGT2385600.017.5064181
AACATAG1141300.017.4578743
AAAGCAA1424300.017.4436151
CCACCTA1885250.017.4360031
ATGGGAG1267550.017.3716641
ACGGAGA1062550.017.3514211
CAGAAAT900700.017.3455529
CGCTGGA995850.017.342791
AGAAACG936550.017.2915868
CGAACAT1595500.017.2821561
ACACTGA1540850.017.2704141
TGGCGCA1982550.017.249541
TAGAATA941050.017.2405827
GCAGAAT752200.017.2348736
GCTTCCA1942300.017.2262951
Produced by FastQC (version 0.11.5)