FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350348654
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7328920.20918932943866825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3051400.049.994271
GTATCAA2630350.046.864289
GGTATCA2915000.041.3806848
TGGTATC2919150.040.2333157
GTGGTAT3056950.038.739286
AGCAGTG4136500.036.0976262
CAGTGGT3887850.031.4923424
AGTGGTA3931100.030.8654945
ATCAACG3180300.028.4540610-11
GCAGTGG5201350.027.823013
CAACGCA3711350.026.93397912-13
ACGCAGA3759350.025.63244614-15
AGAGTAC2821150.024.74026518-19
TCAACGC3406050.024.59382412-13
CTACACG319150.022.3999611
AACGCAG4173500.021.47076214-15
CGCAGAG4185900.020.67018316-17
CAGAGTA3188250.020.3572318-19
GCAGAGT3797950.019.17954316-17
GTACTTT2648450.018.89057522-23
Produced by FastQC (version 0.11.5)