FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332014298
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1090750.018.2090051
ACTGAAC1438650.018.1656321
CAATGGA1864250.017.8537068
GACTAAC1041550.017.8217771
CCGGGAT1131850.017.6416951
GCGCGAT779000.017.3676011
TACGGAT1188850.017.359811
GTGATAA941650.017.2729438
GGAACTT1211300.017.2545491
GGCTCGA887200.017.1460361
ATAGACC1099800.017.1352541
CCACCTA1336950.017.1329151
CAATGAA674000.017.1021218
GCAGAGA630350.017.0637654
CGGACTG821000.017.0513741
GCTGCGA1627600.017.0203761
TGACTAG937000.017.0117741
TAAATGA708100.016.9522049
GAATGAA698100.016.8760951
CTTAGGA947650.016.8174461
Produced by FastQC (version 0.11.5)