FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332014298
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT7324460.22060676435085333No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6572600.19796135406192658No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG4757180.14328238357975775No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT4675110.14081050208265428No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC4534140.13656460060042355No Hit
GTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAG4400220.13253103937108154No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT4249740.12799870444133704No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4089660.12317722533744616No Hit
GGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACG3854600.11609740975673283No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT3752630.11302615648197176No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG3706430.1116346501438923No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA3695930.1113183987034197No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG3628070.10927451082242247No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC3581820.10788149852510268No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC3338200.10054386272244215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1897000.052.5942279
GTGGTAT1997850.048.6209536
GGTATCA2073100.047.6029138
TGGTATC2061200.046.29177
AAGCAGT2441900.046.1444051
AGCAGTG3305500.034.0444762
CAACGCA2051600.033.16092712-13
ATCAACG2037750.032.59901410-11
TCAACGC2056250.030.24917612-13
GCAGTGG3873600.028.3072593
ACGCAGA2374350.027.80049714-15
AGAGTAC1972450.026.34187518-19
CAGTGGT3742100.026.32114
AACGCAG2594200.024.8444514-15
ACGACGC281600.023.053675
CGCAGAG2636600.022.92944716-17
CAGAGTA2186900.022.67006118-19
GCAGAGT2456350.022.07049816-17
TATCAAC2400100.022.04200710-11
AGTGGTA4791600.020.5377775
Produced by FastQC (version 0.11.5)