FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353499731
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT3559640.10069710632962263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT1648750.018.1820141
CCGGTAG1343350.017.8888381
CCGGTAA1125950.017.81641610
GTGATAA1032650.017.7309158
GTAACTG1835450.017.6834031
TATTGCA1460750.017.4953633
AGAGTAA1048750.017.4896668
CCTGGAG1213750.017.473063
GAAGATA618400.017.4489526
CTGATAG1153500.017.4222621
AGACTAG1218500.017.3696618
AGAAGAT819950.017.3634438
GACCTGG1746150.017.290261
ACCTGGA1276350.017.2581732
AATAACA796350.017.2522953
GAAGGTA968450.017.205194
GGTATTG1801700.017.169191
TGGCTGG1770200.017.1520861
TTTAAGA621250.017.1306174
ATGAGAA697300.017.1162575
Produced by FastQC (version 0.11.5)