FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353499731
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5763730.1630476488255093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1973650.044.4757549
AAGCAGT2631400.041.189571
GGTATCA2249550.038.5486838
TGGTATC2372400.035.6807257
GTGGTAT2510300.034.067446
AGCAGTG3390400.031.4914572
ATCAACG2084150.029.80177110-11
AGTGGTA3060200.028.7634265
CAGTGGT3154800.027.8670564
CAACGCA2554750.026.23771712-13
TCAACGC2324900.025.27633512-13
AGAGTAC1998300.024.13884418-19
ACGCAGA2766950.023.64491814-15
GCAGTGG4558100.022.5015963
AACGCAG2966650.021.10669914-15
CGCAGAG3180750.018.81819216-17
TATCAAC2594150.018.2244510-11
GCAGAGT2957050.017.32754316-17
CAGAGTA2659000.017.28094318-19
GTACTTT2283750.016.69152322-23
Produced by FastQC (version 0.11.5)