FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345211222
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG1263400.018.2299825
CGGACTG1627700.018.1346661
TAACTGA971500.017.9381642
GCCAGAA2075700.017.81663710
GACTAAG963450.017.7861379
GACTGCG2153350.017.7759591
AGACTAA969700.017.6785438
TATGAAA730300.017.6515399
CGCTGGA1082750.017.6044161
GGGACCT1329000.017.5819681
CCTACAC2287500.017.5548151
TGACGGC1525150.017.5227831
CCGGAAA635500.017.51242610
CAGGAAT1696050.017.495668
ATTGGAC1508850.017.4908771
AGGGTGA2184200.017.4813561
AGCCAGA2117650.017.4747569
TAGACTA1006100.017.4723747
AACTGAG994900.017.4504223
CATCGAA1205400.017.4498521
Produced by FastQC (version 0.11.5)