FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345211222
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT5200210.15063849807292765No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT4434120.1284465775565083No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4421340.12807636942926498No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4333690.12553734420603513No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4202350.12173271702042177No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA4156030.12039092981745536No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT4123600.11945150496874635No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC4094010.11859434859275808No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA3545590.10270784302603No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT3543080.10263513391809725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1522600.049.2843979
GGTATCA1754950.042.362128
TGGTATC1817350.038.8051537
GTGGTAT1913300.037.3974846
AAGCAGT2285550.036.8973961
ATCAACG1642400.031.28448710-11
CAACGCA1799650.029.5815712-13
AGCAGTG3023000.028.043572
TCAACGC1804350.026.90128912-13
ACGCAGA1990450.025.35039914-15
AACGCAG2128250.023.55521214-15
CAGTGGT3221750.022.7802624
AGAGTAC1687800.022.14137818-19
CTACACG274600.021.0650981
GCAGTGG4196050.020.1722933
CGCAGAG2340000.019.79699116-17
ACGACGC292250.019.2390545
TATCAAC2120250.018.87979510-11
CAGAGTA1972800.018.13396518-19
GTACTTT1726400.018.13218322-23
Produced by FastQC (version 0.11.5)