FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359359810
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT3730560.10381127483343226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA2480150.018.9234491
CTCGAAA1406400.018.610961
CGATTGA2573500.018.566061
ACGAGGA1323700.018.2865681
AGCTAGA1064700.017.8587024
GATGAAA863650.017.711031
CTGAAAC1327850.017.6550431
CCGGGAT1171800.017.5445611
AAGGAAG879350.017.47322710
ACTGAAC1121250.017.4162451
AGATAAT1261450.017.412969
GAATAAG1686600.017.392381
ATCCGAA1777350.017.3731351
GAGCGAA680150.017.34262
GACTAAC949600.017.3340641
ACATATA1420150.017.3297887
TGATCTA1391650.017.3008545
GTTTCTA1784250.017.2338751
TGGGAAG1153500.017.2131711
CATATAC1891600.017.2103428
Produced by FastQC (version 0.11.5)