FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359359810
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT5716400.15907176709604784No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA5509060.15330206235360597No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5487380.1526987672884177No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT5417920.1507658855897102No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT5298460.14744164073328067No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG5232800.14561450263456005No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC5087830.1415803842950607No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA4652040.12945354128498676No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA4129760.1149199182846852No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT4007540.1115188701819494No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA3776650.10509383339222045No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT3734980.1039342713365749No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT3648490.10152749134634727No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG3633640.10111425648850383No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3628500.10097122435588998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1258800.050.1014949
GGTATCA1605600.038.854358
TGGTATC1656250.036.3966527
GTGGTAT1722500.035.816546
AAGCAGT2099700.033.8574181
ATCAACG1343800.031.39753710-11
CAACGCA1477250.029.35128412-13
TCAACGC1493050.026.89110812-13
AGCAGTG2800800.025.5443842
ACGCAGA1672150.024.82981114-15
AACGCAG1802300.022.86910814-15
CTACACG264950.021.207551
CAGTGGT2978750.021.024214
AGAGTAC1454750.020.93917518-19
CGCAGAG1978800.019.38065116-17
ACGACGC286100.019.1564855
AGTGGTA3500350.018.0617085
GCAGTGG4021200.017.662893
CAGAGTA1725500.017.07782618-19
TATCAAC2058200.016.41892810-11
Produced by FastQC (version 0.11.5)