FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358514698
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACAAC2459200.018.5755081
TGCCAAA1442100.018.059821
TATTGAG1362200.018.0115343
GAAAAGA686400.017.9878444
AAAAGAG590500.017.9580785
CAATGGA1338300.017.8948278
ATAGACC1649300.017.8918931
CCACTAC2185400.017.8768561
GCCGAAG951200.017.8687253
CACACAA1558200.017.8248021
AGAAAAG647300.017.6972873
TAGAAAG885200.017.6475439
CGATTGA1326350.017.6053891
CAATGAA768800.017.5879348
GCGCCAA1272250.017.5779781
AAGAAAT616050.017.5581462
CCGGGAT1190450.017.5222551
AGAAAGG1103700.017.49887710
AAGAAAA660200.017.4037742
CTCGAAA739650.017.3758431
Produced by FastQC (version 0.11.5)