FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358514698
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7713840.21516105317389245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1717750.051.6977929
GGTATCA1933400.045.8422168
GTGGTAT2026050.043.1513566
AAGCAGT2296150.041.749061
TGGTATC2123900.041.066627
ATCAACG1604350.033.18639810-11
CAACGCA1661450.032.82248712-13
AGCAGTG3027550.031.6247792
TCAACGC1673550.030.1386912-13
AACGCAG1947350.026.54979514-15
ACGCAGA2147950.025.03879514-15
AGAGTAC1943950.023.9934218-19
CAGTGGT4064100.022.161294
GCAGTGG4391150.021.6781523
CGCAGAG2408550.020.81786316-17
GTACTTT2046800.020.4445322-23
AGTGGTA4532950.020.433575
CAGAGTA2349050.018.9359318-19
TATCAAC2528650.018.42558110-11
CTACACG217750.018.1917081
Produced by FastQC (version 0.11.5)