FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360577037
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA2064050.018.1724341
ACTGATG1615000.017.9184421
ATGCGAT1312600.017.8855461
AGACTAA950250.017.8726548
CGGACTG1436700.017.8424911
CCACCTA1809600.017.8020131
CTCGAAA772800.017.6577011
TGACTAG1388450.017.6269951
GACTAAG967750.017.5449499
CAATGAA712500.017.5407528
GAAGACT1178100.017.532866
CAATGGA1424050.017.5289638
TAGAGAA707000.017.5238865
GGAAGAC1153600.017.5169325
CCTAAGA964000.017.5028554
GAATGAA962300.017.4079911
ATGGGAG1042500.017.400171
CGAGGAA621200.017.3112552
GAAATGA1066050.017.2880741
ATAGAAA818500.017.2832229
Produced by FastQC (version 0.11.5)