FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360577037
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6691150.1855678347037945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1749100.046.5792739
GTGGTAT2063600.038.6718676
AAGCAGT2338400.038.5663071
GGTATCA2259050.035.9269648
TGGTATC2388300.033.194037
CAACGCA1621700.032.31722612-13
ATCAACG1662150.030.72381810-11
TCAACGC1629350.029.68485312-13
AGCAGTG3136500.028.602292
AACGCAG1989100.024.9208714-15
AGAGTAC1771800.024.142718-19
ACGCAGA2145850.024.00382214-15
ACGACGC238050.020.8039055
CAGTGGT3986350.020.5988584
CGCAGAG2373300.020.16865516-17
GCAGTGG4397450.020.1250883
AGTGGTA4223750.019.7386195
TATCAAC2240450.019.1384110-11
CTACACG268800.018.614281
CAGAGTA2196550.018.58322318-19
Produced by FastQC (version 0.11.5)