FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365467923
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTTTTACTTCCTCTAGATAGTC4601150.12589750592147045No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4238190.11596612816824418No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCC3972930.10870803564339078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCT3639100.018.863231
AAAGAAA1149150.018.8313756
AGTGAGA1623900.018.80300510
CTAAGAG1900700.018.712255
CGTTAGA2215700.018.7075061
AGAGACT2390000.018.619018
GCAGGAA1569250.018.6020267
GAATGAA1128300.018.5965421
AGGGTGA2894100.018.5915831
CCTAAGA1931450.018.5598244
AGAAACC2151550.018.5095838
CTGCCTA2182350.018.5057851
ACTGATG2008550.018.4930631
GAAAGAA1168150.018.479455
GATGAAA1173350.018.4781461
CGCATGA2424100.018.4594159
GCAGATA2006200.018.4429026
ACTAGTA2138800.018.4240139
AAAGACG1213800.018.4022466
CGAAAGA1232600.018.394134
Produced by FastQC (version 0.11.5)