FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365467923
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5710080.15624025093988891No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4342670.11882492899383676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1556800.049.8531889
AAGCAGT2276550.040.183391
GGTATCA2148500.035.769388
GTGGTAT2294750.033.2157486
TGGTATC2428850.030.941477
ATCAACG1734950.030.0294510-11
AGCAGTG3327900.027.4180722
TCAACGC1839450.026.65106412-13
CAACGCA2131700.025.33265312-13
ACGCAGA2181950.024.27583514-15
AGAGTAC1803950.023.3564718-19
AACGCAG2261450.022.59957914-15
AGTGGTA3575600.022.1412185
CAGTGGT3742700.021.0110554
GCAGTGG4182900.020.9393623
CGCAGAG2494900.019.5111516-17
CAGAGTA2340300.017.15768218-19
TATCAAC2460400.016.97499710-11
AGTACTT2330200.016.69872520-21
GAGTACT2332350.016.12496220-21
Produced by FastQC (version 0.11.5)