FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394055758
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT5636190.14303026629038626No Hit
CCACCTACTAAGAACGGCCATGCACC4929480.12509600227691633No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG4681390.11880019273820634No Hit
TTCGGTCTTGATTAATGAAAACATTC4282820.10868563428021269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG1739600.018.0124321
AAACCTG2002000.017.9207041
GACTAAC1212750.017.8968371
AGAAGAA958250.017.6868348
CTGATAG1536500.017.6271081
CGCTATC2227950.017.5808561
GGAAAGC1064800.017.5043431
CCGGGAT1201900.017.4870871
GACCAAT1198500.017.4344731
AAACCGA871000.017.4175742
CAATGAA800050.017.4009028
TACGGGC2265850.017.1760351
TAAACCG1678200.017.0895441
AAGAAGC992450.017.05075310
TACGGTA1774850.017.0440271
ACGCCGA1480900.017.0226381
ACTGAAC893950.017.02131
AAGAAGG735050.017.0129767
CTGAAAC1069400.017.0022141
CCCGAAA597850.016.97991410
Produced by FastQC (version 0.11.5)