FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394055758
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA908250.034.179989
ATCAACG839950.025.34191310-11
GGTATCA1328250.023.4101528
CAACGCA999000.022.50175512-13
TGGTATC1384250.021.9764257
TCAACGC974700.020.75200512-13
GTGGTAT1497700.020.7497846
AAGCAGT1908150.020.3882471
CTACACG274050.018.6708281
ACGACGC276400.018.3334145
ACGCAGA1420700.016.0226714-15
AACGCAG1491550.014.71372814-15
TACACGA356300.014.4419432
AGAGTAC1271750.013.8589818-19
AGCAGTG2844000.013.6302082
CGCAGAG1849650.011.40253916-17
CAGTGGT2987400.011.0753484
AGTGGTA3189950.010.7437945
CACGACG504150.010.5096074
GTACTTT1454250.010.24642822-23
Produced by FastQC (version 0.11.5)