FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337416474
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG1340850.017.8530941
CGATTGA1611050.017.7036191
ACTGAAC982150.017.6492771
ACGAGGA1070100.017.554841
GACTAAC857000.017.5082931
AAATGAG705550.017.4805562
GCGCGAT930150.017.4490761
AAGAAGG1066550.017.3458567
GAAATGA1290700.017.3319871
GAATGAA674050.017.3072661
ACACCGG1770400.017.273491
CAATGGA1134100.017.2464318
CTAGAAG618600.017.2153786
CGCTATC1486200.017.2019941
GATGAAA668000.017.185611
GGGAGAT742300.017.1618941
TAAAGAA476100.017.1283345
AGATATG1207250.017.1174938
ACGCCGA1294100.017.1022281
TCTGGAA933300.017.026631
Produced by FastQC (version 0.11.5)