FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337416474
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6392620.18945785083392225No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3622920.10737235076435539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1736700.048.2880449
GTGGTAT1903550.043.1452676
AAGCAGT2287900.040.9584431
GGTATCA2122350.039.390928
TGGTATC2212050.036.692737
ATCAACG1690450.031.6566310-11
CAACGCA1765600.031.3155212-13
TCAACGC1705900.029.80070912-13
AGCAGTG3211100.029.5975882
ACGCAGA2079550.025.9542114-15
AACGCAG2106900.024.95467614-15
AGAGTAC1791150.024.4753918-19
CAGTGGT3575600.023.6635424
GCAGTGG4156800.022.2978123
ACGACGC219950.021.2065545
TATCAAC2090150.021.10910810-11
CGCAGAG2461850.020.36103416-17
CAGAGTA2173800.019.43211618-19
AGTGGTA4515350.019.1035
CTACACG266200.018.1323741
Produced by FastQC (version 0.11.5)