FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377970680
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1380600.018.5680141
GGAACTT2556650.018.4101161
CAATGAA1061350.017.9541038
GACGGCT2278900.017.913871
TGACTAG1536000.017.8730491
ATGAAGA891700.017.7872264
AAGACTA1072300.017.7206487
ATGCGAT1363250.017.7058321
ATGGGAG1395400.017.6501851
GAATGAA986400.017.6254561
ACGCCGA2339300.017.6010171
AAATGAA875250.017.5721822
CTAAACA1133200.017.5587610
GATGAAA812250.017.508841
GCTAGAA894300.017.4509375
AAGCTAA956750.017.4409377
CTGCCTA1388800.017.400751
CCTAGAA1588800.017.3115449
CGCCGAA742400.017.2961372
TGTGAAA1082900.017.2662739
Produced by FastQC (version 0.11.5)