FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377970680
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1001300.033.1172879
ATCAACG890800.026.41314710-11
GGTATCA1337650.024.8655388
CAACGCA1062250.023.6128412-13
GTGGTAT1450050.022.394996
AAGCAGT1852500.021.7355231
TGGTATC1491550.021.4740547
TCAACGC1087550.020.6435412-13
ACGCAGA1464500.017.35016414-15
CTACACG252350.017.1061861
ACGACGC251550.016.800965
AACGCAG1520700.016.25594314-15
AGCAGTG2619600.015.7974812
AGAGTAC1231200.015.38027618-19
TACACGA329700.012.8200982
CGCAGAG1839300.012.74665316-17
CAGTGGT2728150.012.716364
AGTGGTA3055600.011.88475
CAGAGTA1684950.011.08900318-19
GTACTTT1432600.010.8653422-23
Produced by FastQC (version 0.11.5)