FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366725894
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7513580.2048827236617221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1680550.051.9750189
GGTATCA2080900.041.8700188
AAGCAGT2289050.041.5671121
GTGGTAT2203150.039.0889556
TGGTATC2468350.034.928817
CAACGCA1663150.032.6700912-13
AGCAGTG3123450.030.3791222
TCAACGC1776650.028.45644812-13
ATCAACG1895800.028.14403510-11
AACGCAG1946550.026.33767114-15
ACGCAGA2038150.026.18022214-15
CAGTGGT3364900.026.1098674
AGAGTAC1791200.025.4003918-19
GCAGTGG4057000.023.0289963
CGCAGAG2274550.021.83244916-17
AGTGGTA4212950.021.270315
CAGAGTA2147050.020.23117618-19
GTACTTT2147600.019.16616822-23
ACGACGC248150.019.1652815
GCAGAGT2643050.017.94945316-17
Produced by FastQC (version 0.11.5)