FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341459794
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAGA673050.017.9298822
CTCGAAA946350.017.9028071
GACTACA1927550.017.7817861
CGGAGCT1333850.017.6361161
GGAATAA1715450.017.6221051
ACACCCT2417650.017.5755141
GGCGACT1418050.017.5607411
TGACTAG1080050.017.4813211
AATAACA860850.017.3869723
GGGATGA1025100.017.3271521
CTGAAAC960250.017.2008971
CGGCTAG1229400.017.134941
CTTAGGA831050.017.0998551
CAATGAA610350.017.0884828
TGTGATG1273200.017.0834737
GGGAATG842250.017.049321
CCGTGGA909350.017.0235861
GCGCGAT788750.017.0201361
AGCTAGA733150.017.0116924
TTGAAAG609100.017.0036853
Produced by FastQC (version 0.11.5)