FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341459794
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5745470.16826197698695972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2043950.041.4314429
GGTATCA2086000.040.220678
AAGCAGT2528800.039.7437931
TGGTATC2089700.038.7819187
GTGGTAT2126550.038.450146
AGCAGTG2998600.033.3658562
ATCAACG1852900.031.60152810-11
AGTGGTA2810550.029.7500825
CAACGCA2132000.029.0722512-13
TCAACGC2050250.027.06394612-13
CAGTGGT3172800.026.5779674
ACGCAGA2285600.025.97056414-15
AGAGTAC1885150.023.74613618-19
CTACACG214050.023.3845331
TCCGATC666650.022.3135266
AACGCAG2620200.022.05216414-15
GCAGTGG4528150.021.6248513
CGCAGAG2516450.021.41434716-17
ACGACGC252800.019.9396515
GCAGAGT2464200.019.05147216-17
Produced by FastQC (version 0.11.5)