FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364040016
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTAAGTTTCAGCTTTGCAACC5198520.14280078484558686No Hit
TTTCCTCTAAGTTTCAGCTTTGCAAC4321030.1186965665884379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACT4656000.019.0579281
CGTAGGC5001000.018.9363731
TCTGGAA3679650.018.9081861
ATAACGC2837350.018.855691
ATGCGAT2147000.018.7564751
TGTAATG4775100.018.7124149
GTAATGA4778600.018.6913510
GTGTAAT4785550.018.6826068
TTGGAAC2163850.018.6703971
TTGTGTA4741200.018.6553786
GTATCTT6302500.018.4780141
GGCGACT1463250.018.4726681
TGTACAG3868450.018.468837
CGCTGGA1412250.018.4632451
CGTAGAC3282050.018.4346922
TAAGAAA1683950.018.4034886
AGAGCGA1332750.018.3892841
GATCGTA2497400.018.3738421
CCTATTA2505200.018.3369581
TGTGTAA4860550.018.3361427
Produced by FastQC (version 0.11.5)