FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364040016
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT7403780.20337819125906204No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT7014220.19267716986365585No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC6264320.17207778608602192No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT5712210.15691159622408102No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA5686820.15621414542515566No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG5631980.15470771762629523No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG5380590.1478021581012127No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT5347710.14689896069007974No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA4866060.13366827233630274No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4708470.12933935262765178No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT4504820.12374518739720086No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA4374150.12015574683416122No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT4152220.11405943900409014No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC3914040.10751675167490378No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA3857740.10597021839489205No Hit
GGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACG3786100.1040023028677155No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3766790.10347186667522836No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC3672140.10087187777730457No Hit
GCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG3641160.10002087243068354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1198700.044.2103469
GGTATCA1478800.035.7180028
GTGGTAT1533450.033.7344676
TGGTATC1520250.033.5998157
CAACGCA1181750.029.41268712-13
AAGCAGT2010050.029.0083081
ATCAACG1179100.028.68449810-11
TCAACGC1218250.026.61896112-13
ACGACGC276250.024.2897595
CTACACG301900.022.7184331
AGCAGTG2654750.021.9720482
AACGCAG1553550.021.18530314-15
ACGCAGA1570750.021.17917314-15
AGAGTAC1401950.019.63071818-19
TACACGA363900.018.5771062
CAGTGGT3201800.016.5895754
CGCAGAG1951050.015.99866116-17
CAGAGTA1674550.015.75582118-19
GTACTTT1543800.015.38945922-23
TATCAAC1872200.015.14958810-11
Produced by FastQC (version 0.11.5)