FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362879664
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC5949280.16394635991505987No Hit
CTGGTCTGTCTTGCGCCGGTCCA4108200.1132110836610563No Hit
GAGTCCGTCTTGCGCCGGTCCAAGA3706880.10215177006998111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCACC1252550.019.52122320
ACTGATG6317050.019.3960721
GAAGAAA3403750.019.2425376
CAGAAGA3430900.019.0692024
TCAATAG6022300.019.0107210
TGAGTCA5953700.019.0050856
GATGAGT5995650.018.9662784
GATGAAA1923050.018.9569281
CCAGCGA2733450.018.9089681
GGAAATA3334350.018.9089610
CTAAGAC4018450.018.890411
ATTACGA2488500.018.8784089
ATGAGTC5997000.018.8569985
GTCAATA6074350.018.8558049
AAGAAAC3542500.018.8502837
TGATGAG6233700.018.8213923
TAACTGA2274250.018.8203562
CTGATGA6244850.018.7989792
AAGACTC3704400.018.7914923
CGGAAAT3355300.018.788999
Produced by FastQC (version 0.11.5)