FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362879664
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT5778250.15923322724416986No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT4972570.1370308257340097No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC4669600.12868177699811803No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4451280.12266545749447122No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4358250.12010179771330476No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT4319270.11902761241533778No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA4216740.11620215785914088No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT3989320.10993506651835966No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3906320.10764780690493585No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA3815760.1051522137652773No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG3756430.10351723650185037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1472000.047.6545879
GGTATCA1740800.039.7220088
TGGTATC1774150.037.5993547
AAGCAGT2354800.033.4566961
GTGGTAT2039850.033.1307036
ATCAACG1636950.029.06160210-11
CAACGCA1812550.027.36076712-13
AGCAGTG3029050.025.9248242
CTACACG290150.025.2613641
TCAACGC1791150.025.235212-13
ACGCAGA1879150.024.72894514-15
ACGACGC317750.022.7767475
AACGCAG2028100.022.548214-15
CAGTGGT3174800.021.7757034
AGAGTAC1636500.021.45645918-19
TACACGA388800.018.8389912
CGCAGAG2279650.018.59350416-17
AGTGGTA3757450.018.4169525
GCAGTGG4333850.017.9205133
CAGAGTA1908050.017.49827218-19
Produced by FastQC (version 0.11.5)