FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348490143
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1304950.018.1664161
CAATGAA851900.017.7827578
ATGCGAT1235800.017.6872121
CTCGAAA770150.017.677861
GATGAAA868550.017.56781
ACTGAAC943550.017.3953671
AGACTAG915200.017.3062868
GGGACCT1340050.017.281421
CCGTGGA847300.017.2236981
GCCTCTA1973800.017.1267811
TGACTAG960950.017.126651
ATAACGC1099650.016.9932381
GGCTCGA954400.016.963671
CAGAAAC804600.016.9453147
ACTGATG984600.016.925981
CCGGGAT897150.016.9009461
ACGAGGA650400.016.8466851
AGGGTGA1174850.016.785951
GCGCGAT774250.016.7663461
AGAAACC1290300.016.703938
Produced by FastQC (version 0.11.5)