FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348490143
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4752240.1363665542758264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1502150.041.2272459
TGGTATC1762000.033.905727
GGTATCA1846200.033.35318
GTGGTAT1819450.033.0673336
AAGCAGT2213600.031.9145181
ATCAACG1351100.029.71387110-11
CAACGCA1480400.028.4143512-13
TCAACGC1468550.026.02195212-13
AGCAGTG3077500.022.9636632
ACGCAGA1845200.022.33574714-15
AACGCAG1783750.022.22617314-15
AGAGTAC1479350.022.03738218-19
CAGTGGT3277300.018.9809994
AGTGGTA3433850.018.3967885
CGCAGAG2208150.017.281616-17
CTACACG311900.016.5812341
ACGACGC305400.016.4891915
TATCAAC2006800.016.46130810-11
CAGAGTA1969650.015.94385618-19
GCAGTGG4398550.015.7445463
Produced by FastQC (version 0.11.5)