FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339491566
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACT1231800.017.696299
GGAACTT1730200.017.6362481
ACTGATG1148900.017.4317021
CCGGGAT1063950.017.3353731
CAATGGA1494500.017.1919028
CACTGAG1179150.017.1737632
TAACACA1494050.017.1388265
GAAATGA1135200.017.0394821
CTGCGGA1282050.016.9786991
TGCCAAA1165200.016.9692841
CGCTGGA1070700.016.9670281
CTAACAC1539250.016.879984
ACACTGA1438300.016.8458391
ATAACGC1339550.016.832771
ATTGCGA788850.016.82293310
TACGGGC1499500.016.8132931
GACTACA1136350.016.8120231
TGAGCCG948500.016.8114071
GCGGGTT1240750.016.8007971
CTCCCGA838350.016.781057
Produced by FastQC (version 0.11.5)