FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339491566
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1330700.041.272759
GTGGTAT1732700.030.108546
AAGCAGT2173800.029.3672491
ATCAACG1448750.027.29792210-11
GGTATCA2005750.027.110018
CAACGCA1569350.026.71785212-13
TGGTATC2030350.025.502957
TCAACGC1535250.023.85656412-13
AGCAGTG2870800.022.2753032
ACGCAGA1780000.022.2632214-15
AACGCAG1881100.020.32924814-15
ACGACGC251650.020.0119745
AGAGTAC1451050.019.85554318-19
CTACACG262850.019.6708831
CAGTGGT2903800.018.6020894
AGTGGTA3059850.017.959075
CGCAGAG2013300.017.4468116-17
GCAGTGG3622050.017.1900983
TATCAAC1792300.016.6247410-11
TACACGA350100.014.5988932
Produced by FastQC (version 0.11.5)