FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339376361
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACAAC2214950.017.8820441
AATAAGA1117600.017.5562362
TCTTGAG1356950.017.3096128
GATGCTA1449000.017.2410181
GGCTCGA1309500.017.1562481
CCTCTGA1193400.017.133991
GGCCCTA858600.017.0869910
GACTAAC1108650.017.0801091
GCCGAAG1008100.017.0387693
CCGGGAT986250.017.0356241
GCCTCTA1558650.017.0100821
AATCCAG2274900.016.9403671
ATGCGAT1040100.016.938561
GGCGACT759000.016.8668581
GACAACA1378950.016.7873942
CGCCGAA774750.016.7401922
ATAACGC1354450.016.7386671
GAACCTA1008600.016.7283421
ATTGGTG1546450.016.7181151
CTGCCTA1107750.016.6917441
Produced by FastQC (version 0.11.5)