FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339376361
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3912140.11527438117588867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1656200.040.6065989
GGTATCA1919250.034.5737848
TGGTATC1897950.032.9137277
GTGGTAT2053050.030.896716
AAGCAGT2615150.030.0673451
ATCAACG1670750.029.1091710-11
CAACGCA1977450.026.18426112-13
AGCAGTG3196250.024.780922
TCAACGC1835100.024.76956212-13
ACGCAGA2149800.022.88625314-15
AACGCAG2169850.021.9256714-15
CAGTGGT2986300.021.7807454
AGAGTAC1687450.020.84714918-19
CTACACG275400.020.6894361
AGTGGTA3199550.020.5293795
ACGACGC299700.018.4218795
CGCAGAG2492250.017.61023916-17
TATCAAC2119900.017.21816810-11
GCAGTGG4611250.016.7730543
TACACGA383050.014.6058842
Produced by FastQC (version 0.11.5)