FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372906197
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1042900.017.9115871
TACACGA1428700.017.632931
GACTAAC1212250.017.6214431
TGCCAAA1243850.017.582631
GGGAATG1082400.017.5162181
CACACAA1509050.017.3595331
CACCGAA818200.017.3208338
ATAGACC1185550.017.2807731
TCGGAAA1067150.017.2162638
CGTTAGA1272400.017.2107071
GAATGAA728900.017.1639821
ATGCGAT969400.017.0627841
CTGCGGA1236600.017.0355591
GAAGCAG2016000.016.9512841
GAAATGA1011150.016.9102421
AGGCGAA595200.016.89703610
TAAATGA852250.016.8656339
TACTTGT1705300.016.8476471
GATCGCG1264800.016.7654651
CCACTAC1408000.016.7459161
Produced by FastQC (version 0.11.5)