FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372906197
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5143030.13791752567737564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1494900.045.665699
GGTATCA1869200.036.408668
GTGGTAT2032450.032.9797676
AAGCAGT2446650.032.3584751
ATCAACG1406750.031.62349110-11
TGGTATC2113350.031.5951847
CAACGCA1502850.031.33780312-13
TCAACGC1479450.028.78373712-13
AGCAGTG3042700.026.0559332
ACGCAGA1853500.024.82429514-15
AACGCAG1885250.023.86736714-15
CAGTGGT2980650.023.0934774
AGTGGTA3061600.022.5364445
AGAGTAC1607550.022.36231818-19
CGCAGAG2179800.019.73630716-17
ACGACGC260850.018.7181825
GCAGTGG4483100.017.412033
TATCAAC2111400.017.19876510-11
GTACTTT1975900.015.77586922-23
CAGAGTA2359700.014.77768718-19
Produced by FastQC (version 0.11.5)