FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334876577
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACA2026100.018.2959673
ATCGAGT1550450.018.061481
GGATTAC1929000.017.7811071
GCAGAGA1371200.017.722244
ACGCCGA2018450.017.5873321
CAATGGA1567850.017.5457768
ATCCGAA1493200.017.5398121
GCAGTTA1452000.017.4080771
CTCGAAA1048550.017.3791331
GACTGCG1740650.017.3783131
CCGGGAT1053150.017.370051
CTGATCC1325100.017.3486941
ATGAGAA1125000.017.34065
ACACCGG1770350.017.332011
GGGACCT1084200.017.331551
GGGAATG1503850.017.3114571
CTTAGGA1021750.017.2946151
ACGAGCC1415650.017.2507971
AATGGAA1177650.017.223319
CGCCGAC1294000.017.1129762
Produced by FastQC (version 0.11.5)