FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334876577
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3858440.11521976348916156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1476600.037.6945089
GTGGTAT1736900.030.5738476
AAGCAGT2170950.030.3019491
ATCAACG1346100.028.73062910-11
CAACGCA1570150.025.95154812-13
GGTATCA2185300.025.1096138
TCAACGC1456150.024.73440412-13
TGGTATC2230050.023.6358837
AGCAGTG2910300.022.3543742
ACGCAGA1784400.021.75604814-15
AACGCAG1821550.020.46135314-15
AGAGTAC1515700.019.51575718-19
CAGTGGT2949400.018.7280084
AGTGGTA2948200.018.7217985
ACGACGC253800.018.649115
CTACACG266250.018.2079871
TATCAAC1749550.017.19619610-11
CGCAGAG2090100.016.67498216-17
GCAGTGG4077800.015.5274633
CAGAGTA2033300.013.862226518-19
Produced by FastQC (version 0.11.5)