FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366901786
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGGGTTGACAGTGAGGGTAATA3830320.1043963301939337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT2149500.018.3664471
ACGAGGA1177850.018.0117681
GACTAAC1143950.017.9408071
GGGAGAT1081750.017.6952191
TGATTTC2021100.017.5782261
CTGATAG1385700.017.4600871
ACTTGAA1039650.017.4168110
AGACTAG1060150.017.383438
ATAGACC1337850.017.3757041
CTGAAAC1159450.017.334981
CAATGGA1205350.017.267858
GACCAAT1161700.017.2359961
GCGCGAT875100.017.2277551
ACAGCCG1561750.017.223241
CGTTGGG2282850.017.1543981
GATGAAA765100.017.1469081
GGAAAGC883400.017.0770651
ATCGAGT1298000.017.0576441
AAGAAAA407500.017.0121122
CTCGAAA631600.017.007271
Produced by FastQC (version 0.11.5)