FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366901786
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7009880.1910560337256031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1833700.050.4354179
GGTATCA2059400.044.5608528
TGGTATC2078700.043.1798977
GTGGTAT2127850.042.3021776
AAGCAGT2559000.040.5717161
ATCAACG1834950.032.80674710-11
AGCAGTG3271950.031.75852
CAACGCA1987950.031.6571312-13
TCAACGC1973950.028.79412512-13
ACGCAGA2246350.027.26940514-15
AACGCAG2424150.024.4196514-15
AGAGTAC2075600.023.70097718-19
CAGTGGT3922300.023.5614814
CTACACG305850.022.2713721
GCAGTGG4566150.022.2696513
ACGACGC305600.021.8886415
CGCAGAG2591850.021.88214116-17
GTACTTT1999450.020.55630122-23
CAGAGTA2311000.020.39046318-19
AGTGGTA4806250.019.3942055
Produced by FastQC (version 0.11.5)