FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347655402
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAGTGCGTAGGGGTTTT4226110.1215603144863545No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCC4126560.11869684682765264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1167700.018.190221
TGCCAAA1437900.018.0677661
GGAAAGC949350.017.8794061
GCCGAAG873300.017.6123283
CACCGAA775950.017.5695698
AAACCTG1313550.017.4469661
ACTGAAC824250.017.4002761
GAAGATA617600.017.3107516
GACTAAC791400.017.301511
CTAATGG1512600.017.2923451
CCGGTAG904100.017.2921161
ACGAGGA991200.017.264981
AGATACA718200.017.2491878
GGCGACT849000.017.2361411
ATGCGAT910900.017.1380941
ACGCCGA1567000.017.1085681
GTATGAA976300.017.0985348
CGCTGGA882850.017.0808471
AGGCGAA499950.017.04213510
AGATATA776650.017.0064058
Produced by FastQC (version 0.11.5)