FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347655402
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8088700.23266429784974263No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4670910.1343545928850546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1857950.059.0729759
GGTATCA2060750.052.570388
TGGTATC2092200.050.432797
GTGGTAT2273250.046.6090476
AAGCAGT2646600.045.6530881
CTACACG403200.043.7395171
ACGACGC492700.035.9127665
ATCAACG1972300.034.4483710-11
CAACGCA2138500.033.41073612-13
TACACGA535650.033.1864662
AGCAGTG3654200.032.8672562
CAGTGGT3363100.032.312644
TCAACGC2089300.031.29633712-13
ACGCAGA2276650.030.03767814-15
CACGACG596000.029.8191784
GCAGTGG4071700.028.902863
ACACGAC644800.027.5053183
AGAGTAC2018300.027.31065218-19
CGCAGAG2371050.026.78661316-17
AACGCAG2629100.025.76025614-15
Produced by FastQC (version 0.11.5)