FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385831169
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1392650.018.2190321
CAATGAA1090900.017.8886098
GATGAAA1084500.017.6387021
CAATGGA1362400.017.6053058
ACTGATG1372400.017.4974881
ACGAGGA1093750.017.3939381
TACGGAT1380500.017.300511
CGGAGCT1397250.017.2971251
GACTAAC1171750.017.265561
CCGGGAT1270450.017.2029041
CATGGCG1891600.017.1124271
GGCTCGA1125350.017.1018451
GGGACCT1452800.017.093341
TCGAGGA781250.017.0740412
ACGGAGA1253900.017.0539571
CCCGAAA632400.017.01086810
GCCTCTA2120100.016.988061
CCGGTAG1898400.016.9612461
CACCGAA782500.016.8839938
AGACTAG986150.016.8498128
Produced by FastQC (version 0.11.5)