FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320376929
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT1632150.017.347991
CGCTTCA1200800.017.2498861
ATGCGAT996350.017.1371121
GTTTCTA1556550.017.0767541
CGGACTG963800.017.051431
ACTGATG949900.016.9872321
GCCGAAG943250.016.9176833
ATAGACC801250.016.8063051
CGCTATC1195650.016.8042621
TTAGGCA1054200.016.804171
AAACCTG967900.016.8007931
ACCCGAG837500.016.79495410
TACGGAT990800.016.7739961
CCGGGAT860500.016.748791
TGGGCGT983200.016.7109641
TAAACCG1393800.016.6559411
TGGACGC1142750.016.6331961
AGCCTAA1182250.016.5507031
AGACGTT1026750.016.5432241
TGCCAAA1025850.016.5009881
Produced by FastQC (version 0.11.5)