FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320376929
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43876801.3695368183019196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5983950.075.634789
GGTATCA6171400.073.27598
TGGTATC6265850.071.831757
AAGCAGT6554050.070.452631
GTGGTAT6761000.066.669986
AGCAGTG7098000.064.885272
CAGTGGT7024250.064.5422744
AGTGGTA7116200.063.7091835
GCAGTGG8202450.056.1230853
ATCAACG6046500.040.2045810-11
AGAGTAC6062150.038.95856518-19
ACGCAGA6361400.038.5584414-15
CAACGCA6453100.038.24983612-13
TCAACGC6247550.037.47107312-13
CGCAGAG6435150.036.5568916-17
AACGCAG6498050.036.37385614-15
AGTACTT6348450.036.14972320-21
GCAGAGT6625650.035.9887516-17
CAGAGTA6572550.034.742918-19
GAGTACT6449650.034.7194120-21
Produced by FastQC (version 0.11.5)